Maxilla has been reported to be involved very rarely. Desmoplastic fibroma is recently being considered

to be one of the oral manifestations of tuberous sclerosis rather than a coincidental finding. We report an unusual case of a girl with a previous diagnosis of tuberosclerosis Etomoxir in vivo who presented at 8 years with an oral lesion diagnosed as desmoplastic fibroma.”
“Paramecia have many benefits in a biology classroom. They provide educational opportunities for students to learn about protists. Viewing paramecia allows students to observe organelles such as contractile vacuoles, food vacuoles, oral grooves, trichocysts, and macronuclei. The organism can be cultured easily and inexpensively using a plastic food container, wheat seeds, brewer’s yeast, a standard aquarium, and a tank heater. An individual culture can typically last 2 to 3 weeks, and sometimes up to a month. Using this simple culturing method, a constant, on-demand supply of paramecia can be available in any biology classroom. This, coupled with the ability to concentrate paramecia, opens the door to a wide variety of laboratory applications.”
“Chlorthalidone (CTD) is an antihypertensive ML323 solubility dmso drug and exhibits four crystalline forms: I, II, III and IV. In this paper, the incidence of CTD polymorphs in raw materials and in tablets as well as the solubility and dissolution properties of forms I and II have been studied. Raw materials

were named as A, B, C, D, and E and tablets as Reference, G1, G2 and S. Using powder X-ray diffraction and infrared spectroscopy analyses we found that A, B, E, Reference and G1 contain CTD form I; C, D and S contain predominantly form II; and G2 contain a mixture of both forms. Solubility experiments showed that form H is up to 49% more soluble than form land dissolution studies showed a significantly effect of the polymorphism on the dissolution of CTD from tablets. Based on these results, it was concluded that only the CTD form I is acceptable for preparation of tablet form. Moreover, we proposed the polymorphic quality control of CTD raw materials

and tablets. (C) 2013 Elsevier B.V. All rights reserved.”
“Staphylococcus aureus belongs to one of the most common bacteria causing healthcare and community associated Raf inhibitor infections in China, but their molecular characterization has not been well studied. From May 2011 to June 2012, a total of 322 non-duplicate S. aureus isolates were consecutively collected from seven tertiary care hospitals in seven cities with distinct geographical locations in China, including 171 methicillin sensitive S. aureus (MSSA) and 151 MRSA isolates. All isolates were characterized by spa typing. The presence of virulence genes was tested by PCR. MRSA were further characterized by SCCmec typing. Seventy four and 16 spa types were identified among 168 MSSA and 150 MRSA, respectively. One spa type t030 accounted for 80.

The objective of this study was to modify an existing procedure for plasma utilizing solid phase extraction/gas chromatography, and extend its use for measurement of structurally diverse mono- and sesquiterpenes in three fluids (serum, plasma, and rumen fluid) from sheep. Selleckchem Epacadostat Generally, terpene recovery was lower from rumen fluid than from serum or plasma, although the extent and direction of differences varied among chemicals. Fourteen terpenes (camphene, beta-pinene, alpha-terpinene, p-cymene, cis-beta-ocimene, 1,8-cineole, gamma-terpinene, terpinolene, linalool, camphor, longifolene, beta-caryophyllene, alpha-humulene, and caryophyllene oxide) were recovered from serum at near unity. Recovery

from rumen fluid was lower than that for serum or plasma for most terpenes, but eight (p-cymene, 1,8-cineole, cis-sabinene hydrate, terpinolene, borneol, terpin-4-ol, alpha-terpineol, and caryophyllene oxide) were recovered at near unity. Yet, 15 terpene recoveries were below 0.75 ng/ng (tricyclene, alpha-pinene, camphene, sabinene, beta-pinene, myrcene, 2-carene, 3-carene, alpha-terpinene, cis-beta-ocimene, limonene, gamma-terpinene, longifolene, beta-caryophyllene, and alpha-humulene). Oxygenated monoterpenes were typically recovered in greater quantities and hydrocarbon monoterpenes were least effectively recovered with this method. This procedure

allows for simultaneous measurement and recovery adjustment https://www.selleckchem.com/products/mln-4924.html of a number of terpenes from serum, plasma, and rumen fluid of sheep. Published by Elsevier B.V.”
“DPP-4 (Dipeptidyl Peptidase-4) inhibitors present a new therapeutic alternative in the field of

oral anti-diabetic therapy. By targeting the incretin system, they lead to glucose-dependent insulin secretion and glucagon suppression.\n\nThe currently available DPP-4 inhibitors, sitagliptin and vildagliptin, have been approved for use in combination with other oral anti-diabetic drugs. Recent studies on these substances have resulted in a mean decrease of haemoglobin A(1c) of 0.74%, which is less than that of substances previously made available. In addition, DPP-4 inhibitors positively influence fasting glucose levels and improve post-prandial glucose utilization, however, no major effects on Nirogacestat ic50 lipid metabolism have been observed so far.\n\nThe main advantage to be seen with DPP-4 inhibitors is that they don’t seem to induce excessive weight gain – something that cannot be said for other anti-diabetics such as sulfonylureas and glitazones.\n\nThe safety profile of these compounds cannot be assessed conclusively from the currently available data. However, overall, DPP-4 inhibitors have been well tolerated in all previously published trials, with low total numbers of adverse drug-induced effects. Further end-point studies and clinical observations are required before a conclusive evaluation of the efficacy-safety profile on these substances can be made.

Koala had the largest RBC mean cell volume (107.7 +/- 2.6 fl) compared to echidna (81.3 +/- 2.6 fl) and humans (88.4 +/- 1.2 fl). Echidna blood exhibited the highest viscosity over the entire range

of shear rates. Echidna RBC were significantly less deformable than koala RBC but more deformable than human RBC. Echidna RBC had significantly lower aggregability (i.e., aggregation in standardized dextran medium) than koala or human RBC, while aggregation in autologous plasma was similar for the three species. Erythrocyte surface charge as indexed by RBC electrophoretic mobility was similar for human and echidna cells but was 40% lower for koala RBC. Data obtained during this preliminary study indicate that koala and echidna have distinct hemorheological characteristics; investigation HDAC inhibitor of these properties may reveal patterns relevant to specific behavioral and physiological features of these animals. (C) 2010 Elsevier GmbH. All rights reserved.”
“Pepper plants showing mottle or severe mosaic patterns were randomly collected from fields in the Chungnam province

of Korea. The major pepper-infecting viruses reported in Korea have been Pepper mild mottle virus (PMMoV), Cucumber mosaic virus (CMV), and Pepper mottle virus (PepMoV); Chili veinal mottle virus (ChiVMV) was first observed in Korea in 2006. To the best of our knowledge, this is the first report on the genomic sequence and phylogenetic LY3039478 in vitro analysis of a ChiVMV

strain from Korea. A Korean strain of ChiVMV (ChiVMV-Kr) was isolated from a diseased hot pepper plant, and its full-length selleck chemicals genomic RNA sequence was determined. The genomic RNA of ChiVMV-Kr was 9 710 nucleotides long, and it encoded a single polyprotein of 3 088 amino acids. The genomic sequence of this isolate is more closely related to the Taiwanese isolate ChiVMV-Ca than it is to the Indian isolate ChiVMV-I. Comparison of the genes of the Korean isolate with those of ChiVMV-Ca revealed that they were 96.7% (nuclear inclusion protein b, = Nib) to 100.0% (protease1, = P1; helper component-proteinase, = HC-Pro; protein of 6 kDa 1 and 2, = 6K1 and 6K2) identical at the amino acid level. The relationship of ChiVMV to other potyviruses was studied by phylogenetic analysis. ChiVMV was found out to be grouped together with Pepper veinal mottle virus (PVMV), Wild tomato mosaic virus (VVTMV), Chili ringspot virus (ChiRSV), and Tobacco vein banding mosaic virus (TVBMV). Other pepper-infecting potyviruses such as PepMoV, Peru tomato mosaic virus (PTV), Pepper severe mosaic virus (PepSMV), Potato virus Y (PVY), and Tobacco etch virus (TEV) were in a different group. The P1 and coat protein (CP) regions of 5 additional Indonesian isolates were sequenced and compared with those of ChiVIVIV-Kr to investigate genetic variability.”
“Hox genes play important roles in the specification of spatial identity during development of vertebrate embryos.

Methods: Between January 2009 and December 2012, 105 COPD patients were screened to participate in the study. 61 patients were randomly assigned into an individualized training group or into a non-individualized training group. Both groups exercised once a week for 60 minutes over a time period of three months. At the beginning

and after three months, the following measurements were performed: 6-minute walking test (6-MWT), health-related quality of life (St. Georges Respiratory Questionnaire; SGRQ and COPD-Assessment-Test; CAT), M. rectus femoris cross-sectional area, and inflammatory markers in peripheral blood. Results: Only in the individualized training group we observed a significant change of the 6-MWT (increase of 32.47 m; p = 0.012)

and the cross-sectional area of the M. rectus fermoris (increase of 0.57 cm(2); p = 0.049), while no significant changes LY2157299 inhibitor occurred in the non-individualized training group. Peroxisome-proliferator-activated receptor-. coactivator 1 alpha increased in the individualized training only after the three months training period (increase of 0.43 relative copies; p = 0.017), all other myokines and inflammatory markers were not influenced by either of the programs. The total drop-out-rate was 44.3%. Conclusion: A low NVP-AUY922 Cytoskeletal Signaling inhibitor frequency outpatient training program may induce modest improvements in exercise capacity and muscle mass only if it is performed on an

individualized basis.”
“Erythromycin (EM) and tobramycin (TOB) are well-known and widely used antibiotics, selleck products belonging to different therapeutic groups: macrolide and aminoglycoside, respectively. Moreover, they possess different solubility: EM is slightly soluble and TOB is freely soluble in water. It was previously demonstrated that PAMAM dendrimers enhanced the pharmacological activity of antifungal drugs by increasing their solubility. Therefore, it appears interesting to investigate the effect of PAMAM-NH2 and PAMAM-OH dendrimers generation 2 (G2) and generation 3 (G3) on the antibacterial activity of antibiotics with different water solubility. In this study it was shown that the aqueous solubility of EM was significantly increased by PAMAM dendrimers (PAMAM-NH2 and PAMAM-OH caused about 8- and 7-fold solubility increases, respectively). However, it was indicated that despite the increase in the solubility, there was only slight influence on the antibacterial activity of EM (2- and 4-fold decreases in the MBC values of EM in the presence of PAMAM-OH G3 and PAMAM-NH2 G2 or G3 for strains of Staphylococcus aureus were noted, respectively). It was also found that there was no influence of PAMAM on the antibacterial activity of hydrophilic TOB.”
“The lymphocyte depleting anti-CD52 monoclonal antibody alemtuzumab has been used in Cambridge, UK, as an experimental treatment of multiple sclerosis since 1991.

The literature contains no reports of the co-existence of bilateral parotid

gland agenesis and Klinefelter syndrome. The condition is usually asymptomatic and causes asymmetry in the head and neck areas. Its diagnosis is generally made in the light of accompanying developmental anomalies. Herein we report the clinical and radiological findings of a 17-year-old male with Klinefelter buy Smoothened Agonist syndrome accompanied by unilateral peripheral facial nerve paralysis and isolated congenital bilateral parotid gland agenesis. Crown Copyright (C) 2009 Published by European Association for Cranio-Maxillo-Facial Surgery. All rights reserved”
“The genetic diversity of wild olive trees sampled from eight different sites from two provinces of Andalusia (Cadiz and Jaen),

Southern Spain, was evaluated by means of agro-morphological traits and SSR markers. The agro-morphological traits BVD-523 in vitro showed a high variation between genotypes and significant correlation coefficients were obtained among the values recorded in two consecutive years, 2007/08 and 2008/09 (r = 0.59-0.78). Traits such as fruit symmetry (position

A), stone shape, fruit flesh/stone ratio and olive oil content on both wet and dry matter, showed the highest values of diversity and were very useful in genotype discrimination. As expected, average values obtained in wild olives Selleck Bromosporine for fruit size and oil content were lower than the previously reported in cultivated material. However, it is noticeable that wild trees with fruit weights (1.3 g) and olive oil percentage in dry matter (33.8%) comparable to the values found in some olive cultivars, were also found. With both DNA-based and agro-morphological descriptors, higher levels of variability were found within each site than between sites. Genetic variation observed among the wild olive germplasm at the DNA level was higher than the agro-morphological traits, indicating the efficiency of SSR markers for detecting genetic diversity among wild olive genotypes and their relationships. The lack of consistency between the relationship studies performed with molecular and morphological markers could indicate that each marker system measures different aspects of the wild olive genetic variability.

The opposite slopes at EC vary dramatically, physically and biotically, representing the dry and hot south-facing slope savannoid-African continent ["African" slope (AS)], abutting with the north-facing slope forested south-European continent ["European" slope (ES)]. African-originated spiny mice, of the Acomys cahirinus complex, colonized Israel 30,000 y ago based on fossils. Genotypically, we showed significantly higher genetic diversity of mtDNA and amplified fragment length polymorphism of Acomys on the AS compared

with the ES. This is also true regionally across Israel. In complete mtDNA, 25% of the haplotypes Cell Cycle inhibitor at EC were slope-biased. Phenotypically, the opposite slope’s populations also showed adaptive morphology, physiology, and behavior divergence paralleling regional populations across Israel. Preliminary tests indicate slope-specific mate choices. Colonization of Acomys at the EC first occurred on the AS and then moved to the ES. Strong slope-specific natural selection (both positive and negative) overrules low interslope gene flow. Both habitat slope selection and mate choices suggest ongoing incipient sympatric speciation. We conclude that Acomys at the EC is ecologically and genetically adaptively, incipiently, sympatrically speciating on the ES owing to adaptive microclimatic natural VX-809 clinical trial selection.”
“During intracranial surgeries,

cranial nerve (CN) X is most commonly monitored with electromyographic endotracheal tubes. Electrodes on these endotracheal tubes may be PD-1/PD-L1 Inhibitor 3 displaced from the vocal folds

during positioning, and there is a learning curve for their correct placement. Cranial nerve XII is most commonly monitored with electrodes in the dorsum of the tongue, which are also prone to displacement because of their proximity to the endotracheal tube. A retrospective review was conducted of a consecutive series of 83 skull base surgeries using alternative sites for monitoring CN X and XII. On-going (spontaneous) and evoked electromyography (EMG) were obtained from the cricothyroid muscle for CN X and submental genioglossus for CN XII. Stimulation of CN X or XII evoked specific compound motor action potentials from these muscles, and well-defined on-going EMG was observed during tumor resection in the vicinity of CN X and XII. Volume-conducted responses from the adjacent platysma muscle during CN VII stimulation were identified by concomitant responses from the orbicularis oris and oculi. In conclusion, during skull base surgeries, CN X may be monitored with electrodes in the cricothyroid muscle and CN XII with electrodes in the submental genioglossus. These alternative sites are less prone to displacement of electrodes compared with the more commonly used EMG endotracheal tube and electrodes in the dorsum of the tongue. The cricothyroid muscle should not be used when the recurrent laryngeal nerve is at risk.

Binary and multinomial logistic regression were used to compare the prevalence of self-reported chronic medical conditions among jail (n = 6582) and prison (n = 14 373) inmates and non-institutionalised (n = 76 597) adults after adjusting for age, sex, race, education, employment, the USA as birthplace, marital status check details and alcohol consumption. Prevalence

and adjusted ORs with 95% CIs were calculated for nine important chronic conditions.\n\nResults: Compared with the general population, jail and prison inmates had higher odds of hypertension (OR(jail) 1.19; 95% CI 1.08 to 1.31; OR(prison) 1.17; 95% CI 1.09 to 1.27), asthma (OR(jail) 1.41; 95% CI 1.28 to 1.56; OR(prison) 1.34; 95% CI 1.22 to 1.46), arthritis (OR(jail) 1.65; 95% CI 1.47 to 1.84; OR(prison) 1.66; 95% CI 1.54 to 1.80), cervical cancer (OR(jail) 4.16; 95% CI 3.13 to 5.53; OR(prison) 4.82; 95% CI 3.74

Alvocidib to 6.22), and hepatitis (OR(jail) 2.57; 95% CI 2.20 to 3.00; OR(prison) 4.23; 95% CI 3.71 to 4.82), but no increased odds of diabetes, angina or myocardial infarction, and lower odds of obesity.\n\nConclusions: Jail and prison inmates had a higher burden of most chronic medical conditions than the general population even with adjustment for important sociodemographic differences and alcohol consumption.”
“Nursing continues to struggle

to integrate the theoretical and clinical aspects of nurse education for the development of competent practitioners. Collaborative approaches to nurse education as well as the development of joint clinical-academic roles have had questionable success to date on merging the theoretical and clinical components of nursing. Lecturer practitioners’ posts are new in Ireland. These posts were created as nurse educators were cognizant of the need for students to integrate theory Navitoclax cost to practice. The aim of the lecturer practitioner role was to increase the support forstudent nurses in both academia and practice. An evaluation of the impact of the lecturer practitioner role from three key perspectives was conducted in three Irish teaching hospitals in 2007. The results of this study suggest that as lecturer practitioners have both a clinical and academic remit they can integrate theory to practice through collaborative practice.”
“The finite element analysis is an accepted method to predict vertebral body compressive strength. This study compares measurements obtained from in vitro tests with the ones from two different simulation models: clinical quantitative computer tomography (QCT) based homogenized finite element (hFE) models and pre-clinical high-resolution peripheral QCT-based (ER-Nor) hFE models.

Diagn. Cytopathol. 2015;43:153-157. (c) 2014 Wiley Periodicals, Inc.”
“Brown Compound Library concentration adipose tissue (BAT) thermogenesis relies on blood flow to be supplied with nutrients and oxygen and for the distribution of the generated heat to the rest of the body. Therefore, it

is fundamental to understand the mechanisms by which blood flow is regulated and its relation to thermogenesis. Here, we present high-resolution laser-Doppler imaging (HR-LDR) as a novel method for noninvasive in vivo measurement of BAT blood flow in mice. Using HR-LDR, we found that norepinephrine stimulation increases BAT blood flow in a dose-dependent manner and that this response is profoundly modulated by environmental temperature acclimation. Surprisingly, we found that mice lacking uncoupling protein 1 (UCP1) have fully preserved BAT blood flow response to norepinephrine despite failing

to perform thermogenesis. BAT blood flow was not directly correlated to systemic glycemia, but glucose injections could transiently increase tissue perfusion. Inguinal white adipose tissue, also known as a brite/beige adipose tissue, was also sensitive to cold acclimation and similarly increased selleck blood flow in response to norepinephrine. In conclusion, using a novel noninvasive method to detect BAT perfusion, we demonstrate that adrenergically stimulated BAT blood flow is qualitatively and quantitatively fully independent of thermogenesis, and therefore, it is not

Mizoribine research buy a reliable parameter for the estimation of BAT activation and heat generation.”
“Carvacrol represents a very frequent constituent of essential oils and occurs in many kinds of plants. Though human beings comequite often into close contact with this phenol derivative, its biological effects are not sufficiently known. In this paper we investigated the influence of carvacrol given to rats in drinking water on resistance of their liver and testicular DNA against the oxidative agent hydrogen peroxide (H2O2). Carvacrol was dissolved in tap water and given to rats either in concentrations of 30 and 60 mg/1kg/day during 7 days or in concentrations of 15 and 30 mg/1kg/day during 14 days. Control animals were given tap water only. After the given time the rats were sacrificed and hepatocytes and testicular cells were isolated and treated with different concentrations of H2O2 (0-250 mu M, 5 min, on ice). Then the level of DNA lesions was detected by single cell gel electrophoresis. The results of both types of application of carvacrol showed that DNA of cells isolated from carvacrol-treated animals was significantly more resistant to damaging effects of hydrogen peroxide than DNA of control animals. We assume that the observed DNA-protective effects of carvacrol, which was given to rats during a short time of their life, could be associated with an increase of antioxidant activity of liver and testicular cells in these animals.

48). The late hospitalization ( bigger than 3 mo after surgery) rate among those operated by surgeons in the bottom quartile for inflammatory bowel disease surgery volume was nearly 1.5-fold higher than that of those operated by surgeons in the second, third, and top quartiles (3.4/100 person-years [py] versus 2.4/100 py, 2.1/100 py, and 2.3/100 py, respectively; P smaller than 0.05). After multivariate adjustment, the relative incidence ratio for late hospitalization for surgeons in the second, third, and top quartiles were 0.88 (95% CI, 0.83-0.93), 0.88 (95% CI, 0.83-0.94), and 0.87 (95% CI, 0.79-0.94) compared with the bottom quartile, respectively. The 5-year risk of recurrent

surgery was 24.3%, and Crenolanib cell line was not associated with surgeon volume. Conclusions: Low surgeon volumes were associated with increased risk of late hospitalizations after Crohn’s disease surgery. Prospective studies are warranted to elucidate whether this correlation is a late-onset consequence of surgical inexperience or other healthcare utilization factors that are associated with lower surgeon volume.”
“Introduction: Overweight, obesity and physical activity are considered modifiable factors related to development of chronic diseases. Taking into account physical activity patterns in college students, it is necessary to establish its association with the excessive weight in order to prevent and to

decrease the prevalence of future chronic diseases. Objective: The aim of this study was to determine the association between excessive weight and physical inactivity among college students. Material and methods: An analytical cross GSK3326595 chemical structure sectional study was carried out in 2013 among college students from a private university of Bucaramanga, Colombia. Students (n=306) 18 to 25 years old were included using simple random sampling. An interview was realized using a survey that included the International Physical Activity Questionnaire (IPAQ), together with socio demographic variables. An assessment of body composition

was realized DZNeP in vitro in order to measure height, weight, body mass index (BMI), total fat percentage, and waist and hip circumferences. A trained professional in physical activity was in charge of data collection and anthropometric measurements. This study was approved by the university research and ethics committee. Participation was both voluntary and anonymous. All data were analyzed in compliance with the Helsinki Declaration. Written consent was signed by every participant of the study. A descriptive analysis of the studied population’s general characteristics was realized. The association between excessive weight and physical inactivity was estimated using regression logistic models that allows estimate row and adjusted Odds Ratios (OR). Results: An excessive weight was observed in 26.47% of the students, among which 20.26% were overweight and 6.21% obese, according to BMI cutoff. Taking into account the total fat percentage, 12.

\n\nResults: Heart rate was robustly positively associated with (quartiles

of) free T3 (FT3) and T3, both in subjects with TSH levels within reference (0.27-4.2 mu U/L) and in narrow TSH range (0.5-2.5 mu U/L; p <0.0001). FT3 and T3 were negatively associated with left ventricular (LV) end-diastolic volume but positively associated with relative wall thickness. Total T3 (TT3) was associated with enhanced ventricular contraction (as assessed by tissue Doppler imaging). Free thyroxine, FT3, and TT3 were positively associated with Acalabrutinib supplier late ventricular filling, and TT3 was associated with early ventricular filling.\n\nConclusion: We have demonstrated a strong positive association between thyroid hormone levels within the euthyroid range and heart rate, and more subtle effects on cardiac function and structure. More specifically, we suggest a smaller LV cavity size (with increased relative wall thickness), an enhanced atrial and ventricular contraction, and LV relaxation with higher circulating thyroid hormones. These this website results illustrate that variation

in thyroid hormone levels, even within the reference range, exerts effects on the heart.”
“MYC/BCL2 double hit lymphoma (DHL) is a rare, recently recognised and highly aggressive subtype of non-Hodgkin lymphoma, with an affinity to involve the central nervous system and the head and neck either at initial presentation or during relapse. We present a case of 43-year-old woman with MYC/BCL2 DHL relapse in the nasopharynx with extensive spread to

the neck, skull base, and the central nervous system. To our knowledge, this is the first reported case in the literature describing the MRI and CT scan findings and the profound pattern of disease involvement of this rare neoplasm. (C) 2013 Elsevier Ltd. All rights reserved.”
“Background: MLN4924 solubility dmso Previous studies reported high C-reactive protein (CRP) levels in Indigenous Australians, which may contribute to their high risk of cardiovascular disease. We compared CRP levels in Indigenous Australians and the general population, accounting for obesity and other risk factors.\n\nMethods: Cross-sectional study of CRP and risk factors (weight, height, waist and hip circumferences, blood pressure, lipids, blood glucose, and smoking status) in population-based samples from the Diabetes and Related conditions in Urban Indigenous people in the Darwin region (DRUID) study, and the Australian Diabetes, Obesity and Lifestyle study (AusDiab) follow-up.\n\nResults: CRP concentrations were higher in women than men and in DRUID than AusDiab. After multivariate adjustment, including waist circumference, the odds of high CRP (>3.0 mg/L) in DRUID relative to AusDiab were no longer statistically significant, but elevated CRP was still more likely in women than men. After adjusting for BMI (instead of waist circumference) the odds for elevated CRP in DRUID participants were still higher relative to AusDiab participants among women, but not men.