This information is useful for clinicians in choosing suitable drug regimens for treating TB patients. This study also indicated that the automatic addition of PZA in the learn more treatment regimen of MDR-TB patients would have less benefit in Thailand and would increase the risk

of XDR-TB development or render treatment ineffective. Therefore, PZA susceptibility testing in MDR-TB patients should be performed before starting or adjusting treatment regimens. Acknowledgements We would like to thank the Molecular Mycology and Mycobacteriology Laboratory, Drug Resistant Tuberculosis Research Fund, Siriraj Foundation, under the Patronage to Pass HRH Princess Galyani Vadhana Krom Luang Naradhiwas Rajanagarindra, Department of Microbiology, Faculty Selleckchem LXH254 of Medicine Siriraj Hospital for supporting essential facilities in pyrazinamide susceptibility by the BACTEC MGIT 960

PZA system and all staff members for their help. JJ was financially supported by the Siriraj Graduate Scholarship. AC was supported by the Chalermphrakiat Grant, Faculty of Medicine Siriraj Hospital, Mahidol University. The study was funded by the Siriraj Graduate Thesis Scholarship, Siriraj Grant for Research and Development, and Drug Resistant Tuberculosis Fund, Siriraj Foundation, Department of Microbiology, Faculty of Medicine Alisertib Siriraj Hospital. The study was approved by the Siriraj Ethics Committee, Mahidol University. None of the authors has any conflicts of interest to declare. References 1. World Health Organization: WHO Report. Geneva. 2009. 2. Vermund SH, Yamamoto N: Co-infection with human immunodeficiency virus and tuberculosis in Asia. Tuberculosis (Edinb) 2007,87(Suppl 1):S18–25.CrossRef 3. Verma JK, Nateniyom S, Akksilp S, Mankatittham W, Sirinak C, Sattayawuthipong W, Burapat C, Kittikraisak W, Monkongdee P, Cain KP, Wells CD, Tappero JW: HIV care and treatment factors associated with improved survival TB treatment in Thailand: an observational study. BMC Infect Dis 2009, 9:42–50.CrossRef 4. Cain KP, Anekthananon T, Burapat C, Akksilp S, Mankhatitham W, Sirinak C, Nateniyom S, Sattayawuthipong

Orotic acid W, Tasaneeyapan T, Varma JK: Cause of death in HIV-infected persons who have tuberculosis, Thailand. Emerg Infect Dis 2009, 15:258–264.PubMedCrossRef 5. Mankatittham W, Likanonsakul S, Thawornwan U, Kongsanan P, Kittikraisak W, Burapat C, Akksilp S, Sattayawuthipong W, Srinak C, Nateniyom S, Tasaneeyapan T, Verma JK: Characteristics of HIV-infected tuberculosis patients in Thailand. Southeast Asian J Trop Med Public Health 2009, 40:93–103.PubMed 6. Zhang Y, Permar S, Sun Z: Conditions that may affect the results of susceptibility testing of Mycobacterium tuberculosis to pyrazinamide. J Med Microbiol 2002, 51:42–9.PubMed 7. Zhang Y, Mitchison D: The curious characteristics of pyrazinamide: a review. Int J Tuberc Lung 2003, 7:6–21. 8.

Detergent (cholate, alkyl glycoside, Triton X-100) removal of mixed micelles (absorption) Detergent absorption

is attained by shaking mixed micelle solution Idasanutlin in vitro with beaded organic polystyrene adsorbers such as XAD-2 beads (SERVA Electrophoresis GmbH, Heidelberg, Germany) and Bio-beads SM2 (Bio-RadLaboratories, Inc., Hercules, USA). The great benefit of using detergent adsorbers is that they can eliminate detergents with a very low CMC, which are not entirely depleted. Selleckchem BAY 63-2521 Gel-permeation chromatography In this method, the detergent is depleted by size special chromatography. Sephadex G-50, Sephadex G-l 00 (Sigma-Aldrich, MO, USA), Sepharose 2B-6B, and Sephacryl S200-S1000 (General Electric Company, Tehran, Iran) can be used for gel filtration. The liposomes do not penetrate into the pores of the beads packed in a column. They percolate through the inter-bead spaces. At slow flow rates,

the ARS-1620 manufacturer separation of liposomes from detergent monomers is very good. The swollen polysaccharide beads adsorb substantial amounts of amphiphilic lipids; therefore, pre-treatment is necessary. The pre-treatment is done by pre-saturation of the gel filtration column by lipids using empty liposome suspensions. Dilution Upon dilution of aqueous mixed micellar solution of detergent and phospholipids with buffer, the micellar size and the polydispersity increase fundamentally, and as the system is diluted beyond the mixed micellar phase boundary, a spontaneous transition from polydispersed micelles to vesicles occurs. Stealth liposomes and conventional liposomes Although liposomes Acesulfame Potassium are like biomembranes, they are still foreign objects of the

body. Therefore, liposomes are known by the mononuclear phagocytic system (MPS) after contact with plasma proteins. Accordingly, liposomes are cleared from the blood stream. These stability difficulties are solved through the use of synthetic phospholipids, particle coated with amphipathic polyethylene glycol, coating liposomes with chitin derivatives, freeze drying, polymerization, microencapsulation of gangliosides [17]. Coating liposomes with PEG reduces the percentage of uptake by macrophages and leads to a prolonged presence of liposomes in the circulation and, therefore, make available abundant time for these liposomes to leak from the circulation through leaky endothelium. A stealth liposome is a sphere-shaped vesicle with a membrane composed of phospholipid bilayer used to deliver drugs or genetic material into a cell. A liposome can be composed of naturally derived phospholipids with mixed lipid chains coated or steadied by polymers of PEG and colloidal in nature. Stealth liposomes are attained and grown in new drug delivery and in controlled release. This stealth principle has been used to develop the successful doxorubicin-loaded liposome product that is presently marketed as Doxil (Janssen Biotech, Inc.

gingivalis (A) or heat-killed P. gingivalis (MOI:1000) (B) for 24 h. CXCL8 levels were significantly suppressed by viable, but not heat-killed, P. gingivalis. (C) Gingival fibroblasts were stimulated with 50 ng/ml TNF-α for 6 h followed by treatment with viable or heat-killed P. gingivalis (MOI:100) for 24 h. Statistically significant differences compared to the negative control (#) or positive control TNF-α (*) were determined using Student’s t-test (###/***- p < 0.001). CXCL8 degradation is due to Arginine-gingipains To determine if P. gingivalis suppresses TNF-α induced CXCL8 release through Kgp and Rgp activities,

viable Caspase-dependent apoptosis P. gingivalis was incubated for 1 hour with increasing concentrations (0.1, 0.25, 0.5 and 1 mM) of cathepsin

B II inhibitor or Leupeptin, before fibroblast infection. The fibroblasts were pre-stimulated buy CT99021 with 50 ng/ml TNF-α for 6 hours and then incubated for 24 hours with treated or non-treated P.gingivalis. The Rgp inhibitor Leupeptin significantly reversed the P. gingivalis-induced suppression of CXCL8 at all concentrations (Figure 4A), whereas Cathepsin B II inhibitor at 1 mM only slightly changed the CXCL8 level (Figure 4B). Figure 4 CXCL8 degradation is due to Arginine-gingipains. The involvement of Kgp and Rgp in CXCL8 degradation was determined by using Cathepsin B inhibitor II and Leupeptin. Viable P. gingivalis were incubated with the indicated concentration of inhibitor for 1 h prior to treatment of cells. Primary fibroblasts (50,000 cells/well) were stimulated with 50 ng/ml TNF-α for 6 h before the cells were incubated with P. gingivalis for 24 h. CXCL8 accumulation was more efficiently selleck compound restored by Leupeptin (A) than with Cathepsin B inhibitor II (B). The asterisks indicate significant differences compared to cells treated with P. gingivalis, without inhibitor. *- p < 0.05 ***- p < 0.001 (Student’s t-test). P. gingivalis targets a wide range of fibroblast-derived inflammatory mediators To examine if the immunomudulatory

role of P. gingivalis accounts for inflammatory mediators other than CXCL8, a parallel determination of cytokines and LDN-193189 mouse chemokines was performed with a cytokine array (Table 1). Primary dermal fibroblasts (50,000 cells/well) were stimulated with 50 ng/ml TNF-α for 6 h before the cells were incubated with viable or heat-killed P. gingivalis, (MOI:1000), respectively (Figure 5). Non-stimulated fibroblasts were used as a control. TNF-α alone, or in combination with heat-killed P. gingivalis, induced secretion of TNF-α itself, as well as serpin-1, IL-6, CCL2, CCL5, CXCL1, CXCL10 and CXCL8. On the other hand, the levels of these inflammatory mediators, except TNF-α and serpine-1, were markedly suppressed by viable P. gingivalis. Heat-killed P.

In Figure 3d, the scanned area at the center of the image is observed as a shallow GSK2399872A hollow, the cross-sectional profile of which revealed its depth to be approximately 1.0 nm. In contrast, the multiple scans (ten scans) using a Pt-coated cantilever in SOW created a clear square hollow, as shown in Figure 3e,f. The etched depth of the 1.0 × 1.0 μm2 central area in Figure 3f was about 4.0 nm from a cross-sectional profile. The mechanism of inducing the

difference between image (d) and image (f) in Figure 3 is as follows. As mentioned previously, we scanned a cantilever in the contact mode. Taking into account the catalytic activity of metals (e.g., Pt) enhancing the reactions in Equations (1) and (2), we suppose that, at each moment selleck chemicals llc during the scan, a Ge surface in contact with a Pt probe is preferentially oxidized in water in the presence of dissolved oxygen. This is schematically drawn in Figure 4a. Owing to the soluble nature of GeO2, the scanned area exhibits a square hollow, as shown in Figure 3f. In Figure 3b,d,f taken after the ten scans, no this website pyramidal pits such as those shown in Figure 1 are observed. This is because we did not fix the cantilever at only one surface site, but rather scanned it over a micrometer area, which is much larger than the etched depth, as schematically depicted in Figure 4b. Figure 5a,b shows a summary of etched depth as a function of pressing force on the n-type and p-type Ge(100) surfaces, respectively.

Because the plots in Figure 5 slightly fluctuate, it is hard to fit them using a simple straight line or a curve. This is probably due to the difference

in probe apex among the sets of experiments performed. However, Figure 5 clearly indicates that (1) the catalytic activity of metals (e.g., Pt) has a much greater effect on Ge etching than that of the mechanical machining caused by a pressurized cantilever, and (2) dissolved oxygen in water is the key molecule in metal-assisted etching. Namely, it is easy to imagine that the Ge surface was machined mechanically to some extent by the pressed cantilever on Ge. In Figure 5, the etched Idoxuridine depth increases slightly at a larger pressing force even with a Si cantilever in SOW (light gray filled circles) or a Pt-coated cantilever in LOW (gray filled circles). This indicates that the mechanical etching of Ge occurs, but its effect is very small. On the other hand, a drastic increase in etched depth is observed with a Pt-coated cantilever in SOW (blue filled circles) at each pressing force, which is probably induced by the catalytic effect of Pt mediated by dissolved oxygen in water. One may think that the difference in etched depth between the blue and gray (or light gray) filled circles increases with increasing pressing force in Figure 5. This is as if the catalytic effect is enhanced at greater pressing forces. As for the reason for this enhancement, we imagine that the probe apex became blunter at larger forces.

References 1. Legler JM, Ries LA, Smith MA, et al.: Cancer surveillance series [corrected]: brain and other central nervous system cancers: recent trends in incidence and mortality. J Natl Cancer Inst 1999, 91 (16) : 1382–90.CrossRefPubMed 2. Addeo R, Casale

F, Caraglia M, et al.: Glucocorticoids induce G1 arrest of lymphoblastic cells through retinoblastoma protein Rb1 dephosphorylation in childhood acute lymphoblastic leukemia in vivo. Cancer Biol Ther 2004, 3 (5) : 470–6.PubMed 3. Casale F, D’Angelo V, Addeo R, Caraglia M, et al.: P-glycoprotein 170 expression and function as an adverse independent prognostic factor in childhood acute lymphoblastic leukemia. Oncol Rep 2004, 12 (6) : 1201–7.PubMed 4. Steelman LS, Abrams SL, Whelan J, et al.: Contributions of the Raf/MEK/ERK, PI3K/PTEN/Akt/mTOR and Jak/STAT pathways to leukemia. Leukemia 2008, 22 (4) : 686–707.CrossRefPubMed www.selleckchem.com/products/lazertinib-yh25448-gns-1480.html 5. Kuntzen C, Sonuc N, De Toni E, et al.: Inhibition of c-Jun-N-terminal-kinase sensitizes tumor cells to CD95-induced apoptosis and induces G2/M cell cycle arrest. Cancer Res 2005, 65 (15) : 6780–8.CrossRefPubMed 6. Gregorj C, Ricciardi MR, Petrucci MT, et al.: ERK1/2 phosphorylation is an independent predictor of complete remission in newly diagnosed adult acute lymphoblastic leukemia. Blood 2007, 109 (12) Selleck Eltanexor : 5473–6.CrossRefPubMed 7. Milella M, Kornblau SM, Estrov Z, et al.: Therapeutic targeting of the MEK/MAPK

signal transduction module in acute myeloid leukemia. J Clin Invest 2001, 108 (6) : 851–9.PubMed 8. Zhang W, Bae I, Krishnaraju K, et al.: CR6: A third member in the MyD118 and

Gadd45 gene family which functions in negative growth control. Oncogene 1999, 18: 4899–4907.CrossRefPubMed 9. Gupta M, Gupta SK, Balliet AG, et al.: Hematopoietic cells from Gadd45a- and Gadd45b-deficient mice are sensitized to genotoxic-stress-induced apoptosis. Oncogene 2005, 24: 7170–7179.CrossRefPubMed 10. Harada K, Toyooka S, Shivapurkar N, et al.: Deregulation of caspase 8 and 10 expression in pediatric tumors and cell lines. Cancer Res 2002, 62 (20) : 5897–901.PubMed 11. Kornblau SM, Womble M, Qiu YH, et al.: Simultaneous activation of multiple signal transduction CHIR 99021 pathways confers poor prognosis in acute myelogenous leukemia. Blood 2006, 108 (7) : 2358–65.CrossRefPubMed 12. Kharas MG, Fruman DA: ABL oncogenes and phosphoinositide 3-kinase: mechanism of activation and downstream effectors. Cancer Res 2005, 65 (6) : 2047–53.CrossRefPubMed 13. Masera G, Conter V, Rizzari, et al.: AIEOP Non-B ALL trials. Int J Pediatric Hematol Oncol 1999, 6: 101. 14. Bennet JM, Catovsky D, Daniel MT, et al.: The morphological classification of acute lymphoblastic leukemia: concordance among observers and clinical correlation. Br J Haematol 1981, 9 (6) : 1012. 15. Barry OP, Selleck LY2109761 Mullan B, Sheehan D, et al.: Constitutive ERK1/2 activation in esophagogastric rib bone marrow micrometastatic cells is MEK-independent. J Biol Chem 2001, 276: 15537–15546.

Zhang X, Chen Z, Wurthner F: Morphology Selleckchem CHIR99021 control of fluorescent nanoaggregates by co-self-assembly of wedge- and dumbbell-shaped amphiphilic perylene bisimides. J Am Chem Soc 2007, 129:4886–4887.CrossRef 13. Boerakker MJ, Botterhuis NE, Bomans PHH, Frederik PM, Meijer EM, Nolte RJM, Sommerdijk NAJM: Aggregation behavior of giant amphiphiles prepared by cofactor reconstitution. Chem Eur J 2006, 12:6071–6080.CrossRef 14. Dai H, Chen Q, Qin H, Guan Y, Shen D, Hua Y, Tang Y, Xu J: A temperature-responsive copolymer hydrogel in controlled drug delivery. Macromolecules 2006, 39:6584–6589.CrossRef 15. Kuroiwa K, click here Shibata T, Takada

A, Nemoto N, Kimizuka N: Heat-set gel-like networks of lipophilic Co(II) triazole complexes in organic media and their thermochromic structural transitions. J Am Chem Soc 2004, 126:2016–2021.CrossRef 16. Aldred MP, Eastwood AJ, Kelly SM, Vlachos P, Contoret AEA, Farrar SR, Mansoor B, O’Neill M, Tsoi WC: Light-emitting fluorene photoreactive liquid crystals for organic electroluminescence. Chem Mater 2004, 16:4928–4936.CrossRef 17. Xing P, Sun T, Li S, Hao A, Su J, Hou Y: An instant-formative heat-set organogel induced CDK inhibitor by small organic molecules at a high temperature. Colloid Surf A-Physicochem Eng Asp 2013, 421:44–50.CrossRef 18. Xin F, Zhang H, Hao B, Sun T, Kong L, Li Y, Hou Y, Li S, Zhang Y, Hao A: Controllable transformation from sensitive and reversible heat-set organogel to stable gel induced by sodium acetate. Colloid Surf

A-Physicochem Eng Asp 2012, 410:18–22.CrossRef 19. Iwanaga K, Sumizawa T, Miyazaki M, Kakemi M: Characterization of organogel as a novel oral controlled release formulation Anidulafungin (LY303366) for lipophilic compounds. Int J Pharm 2010, 388:123–128.CrossRef 20. Lofman M, Koivukorpi J, Noponen V, Salo H, Sievanen E: Bile acid alkylamide derivatives

as low molecular weight organogelators: systematic gelation studies and qualitative structural analysis of the systems. J Colloid Interf Sci 2011, 360:633–644.CrossRef 21. Bastiat G, Plourde F, Motulsky A, Furtos A, Dumont Y, Quirion R, Fuhrmann G, Leroux JC: Tyrosine-based rivastigmine-loaded organogels in the treatment of Alzheimer’s disease. Biomaterials 2010, 31:6031–6038.CrossRef 22. Tao ZG, Zhao X, Jiang XK, Li ZT: A hexaazatriphenylene-based organogel that responds to silver(I) with high selectivity under aqueous condition. Tetrahedron Lett 2012, 53:1840–1842.CrossRef 23. Miyamoto K, Jintoku H, Sawada T, Takafuji M, Sagawa T, Ihara H: Informative secondary chiroptics in binary molecular organogel systems for donor-acceptor energy transfer. Tetrahedron Lett 2011, 52:4030–4035.CrossRef 24. Marquette C, Blum L: Electro-chemiluminescent biosensing. Anal Bioanal Chem 2008, 390:155–168.CrossRef 25. Sakura S: Electrochemiluminescence of hydrogen peroxide-luminol at a carbon electrode. Anal Chim Acta 1992, 262:49–57.CrossRef 26. Leca B, Blum LJ: Luminol electrochemiluminescence with screen-printed electrodes for low-cost disposable oxidase-based optical sensors.

Poster No. 151 Novel Role of Tumor-Derived ExtracellularHsp90 as an Essential Mediator of Prostate Cancer Cell Migration and Stromal Cell Activation: Evidence for Autocrine and Paracrine Functions Venkatesababa Samanna1, Udhayakumar Gopal1, Jennifer Isaacs 1 1 Department of Cell and Molecular BIRB 796 Pharmacology, Hollings

Cancer Center, Medical Uninversity of South Carolina (MUSC), Charleston, SC, USA Prostate cancer (PCa) is one of the most common and lethal diseases among men. Although early cancer is often curative, subsequent metastatic spread of tumor cells renders the disease untreatable. Treatment failure is also due to a poor understanding of the contribution of the tumor microenvironment to disease progression. We find that a number of PCa cells secrete heat shock protein 90 (Hsp90). This extracellular Hsp90 (eHsp90) acts in a manner distinct from the intracellular chaperone and has been implicated in regulating cell motility selleck chemical in other models. Interestingly, we find that eHsp90 check details expression correlates with cancer aggressiveness.

Consistently, the more aggressive and metastatic PCa cells secreted several fold more eHsp90 relative to their weakly tumorigenic matched counterparts. Interference with this pathway by antibody or drug-mediated neutralization of native eHsp90 dramatically impaired tumor cell migration, thereby implicating eHsp90 in a constitutive pathway culminating in cell migration. Concomitant with inhibition of eHsp90, the activation of downstream mediators such as FAK, Src, and ERK were attenuated. The multifunctional receptor LRP1 (LDL-receptor Related Protein-1) has been proposed as the receptor for eHsp90. We find that silencing of LRP1 similarly reduced PCa signaling and migration, implicating an eHsp90-LRP1 signaling axis in PCa development. Addition of Hsp90 to prostate stromal cells, which lack Hsp90 secretion, potently stimulated ERK activation and cell motility, implicating paracrine effects. ERK activation

was inhibited by pretreatment with an inhibitor of MMP activity, Pregnenolone suggesting that eHsp90 modulates ERK signaling and MMP activity to modulate cell migration. We propose that PCa aggressiveness may be due in part to increased secretion of eHsp90, which then activates the stroma to further support tumor growth. Poster No. 152 IL-6 Promotes Pancreatic Cancer Progression by Intractions of Fibroblasts Hidenobu Kamohara 1 , Takatoshi Ishiko1, Hiroshi Takamori1, Hideo Baba1 1 Department of Gastroenterological Surgery, Kumamoto University, Graduate School of Medical Sciences, Kumamoto, Kumamoto, Japan Introduction: IL-6 has pleiotropic function and are produced by various immunnocompetent cells, as well as cancer cells. Some studies have been demonstrated IL-6 play an important role in evading host immune surveillance in tumor microenvironment, but interactions of fibroblasts has not been fully understood. Therefore, the aim of this study is to reveal role of fibroblasts in pancreatic tumor microenvironment.

Amino acids encoded by DUS are highlighted in purple. The organization of the fpg flanking region is unique for Neisseria species http://​string.​embl.​de/​ (data not shown). Upstream of the fpg gene are the hypothetical ORFs NMB1297 and NMB1296 (Figure 1A). NMB1297 is annotated as an ortholog to mltD http://​www.​ncbi.​nlm.​nih.​gov/​COG/​, which encodes a membrane-bound lytic murein transglycosylase of unknown function. NMB1296 shows 30–40% amino selleck kinase inhibitor acid identity with DNA methyltransferases in a number of bacterial species http://​www.​ncbi.​nlm.​nih.​gov/​blast/​Blast.​cgi. Downstream, fpg is flanked by the nlaA gene, encoding a lysophosphatidic acid acyltransferase involved in

biosynthesis of the glycerophosholipid membrane [26], about 300 bp of non-coding sequence containing two DUS within a predicted

terminator, and the opposite oriented hypothetical ORF NMB1293. The NMB1296, fpg and nlaA genes are all oriented in the same direction and a putative promoter is found upstream of NMB1296 while none are identified between these genes. At the end of NMB1296 a terminator is predicted by TransTermHP. Between fpg and nlaA, a terminator is predicted by GeSTer. This intrinsic terminator contains a DUS and an imperfect DUS as inverted repeat, a structure found in many putative Mc transcription terminators or attenuators [24]. https://www.selleckchem.com/products/BIRB-796-(Doramapimod).html The VIMSS Operon Prediction suggests co-transcription of fpg and NMB1296. However, Swartley and Stephens have evidence by

reverse transcriptase PCR that nlaA and fpg are co-transcribed in Mc KPT 330 strain NMB [27]. In microarray analysis of an MC58 fpg mutant compared to wildtype, nlaA was the only gene significantly down-regulated at least 1.5 fold, supporting the evidence for co-transcription of these two genes (unpublished data). The Mc fpg open reading frame encodes 276 amino acids containing a predicted N-terminal glycosylase catalytic domain, a helix-two-turn-helix and a C-terminal Phospholipase D1 zinc finger (Figure 1B, additional file 1, Figures S1 and S2). These regions contain long sequences with a positive electrostatic charge, enforcing binding to negatively charged DNA (See additional file 1, Figure S3). Alignment of the deduced Fpg sequence from the genomes of five Mc strains reveals non-synonymous or synonymous substitutions in 5 out of 276 amino acid positions (see additional file 1, Figure S1). The positions showing variation correspond exactly to those found in the fpg gene from 11 Mc clinical isolates previously sequenced [10]. An additional 6 amino acids show non-synonymous or synonymous variation when the N. gonorrhoeae and N. lactamica sequences are included in the comparison. All known functional residues exhibit complete sequence conservation (see additional file 1, Table S1 and Figure S1).

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of copper oxides in contact killing of bacteria. Langmuir 2013, 29:16160–16166.PubMedCrossRef 58. Mathews S, Hans M, Mucklich F, Solioz M: Contact killing of bacteria on copper is suppressed if bacterial-metal contact is prevented and is induced on iron by copper ions. Appl Environ Microbiol 2013, 79:2605–2611.PubMedCentralPubMedCrossRef Competing interests KT is an employee of EOS Surfaces. ABM, VK and GB are employees of Cupron Inc. This study was funded by Cupron Inc. and EOS Surfaces that developed the antimicrobial surfaces. Authors’ contributions ABM and GB made substantial contributions to conception, design, analysis and interpretation of data of the study, and writing the manuscript; VK and KT were key in designing and developing the test materials studied, and revising the manuscript critically for important intellectual content. All authors read and approved the final manuscript.