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“MicroRNA biogenesis genes have been confirmed involved in lots of diseases. This study evaluated the role of genetic variants in microRNA biogenesis genes in semen quality and idiopathic male infertility. Seven single-nucleotide polymorphisms (SNP) of DICER1 (rs13078, rs1057035 and rs12323635) and DROSHA (rs10719, rs2291109, rs17409893 and rs642321) were determined by Taq-Man probes and SNPstream in 667 eligible infertile men and 419 fertile controls. Semen quality analysis was performed by computer-assisted sperm analysis. It was found that genetic variants of rs12323635 was associated with idiopathic OSI-906 chemical structure male infertility. Additionally,
in strategy analysis, the rs12323635 C allele might decrease the risk of oligozoospermia (OR 0.42, 95% CI 0.26-0.66; P = 0.0002). The rs642321 TT genotype may have a higher risk of oligozoospermia (OR 2.38, 95% CI 1.34-4.25; P = 0.003). These significant differences were retained after Bonferroni correction. The results showed that variants of DICER1 and DROSHA may modify the risk of abnormal semen parameters, which could result in male infertility. (C) 2012, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.”
“Background: Scoliosis surgery is one of the most painful operations performed. Postoperative pain management has been historically based on the use of intravenous opioids. Many of the adolescents who undergo these procedures are at increased risk for opioid-related side effects
because of underlying medical problems. Epidural analgesia selleck kinase inhibitor has been demonstrated to provide superior pain control with fewer side effects for chest and abdominal surgery in children as well as adults. We aim to analyze the available literature for sufficient evidence to allow recommendations regarding the use of epidural analgesia with parenteral opioids vs. intravenous opioids only.
Search strategy: Public Medline and the Cochrane database were searched (1966-10/2008) using scoliosis-related and epidural analgesia-related terms. In Medline, the intersection of these results was combined
with Phases 1 and 2 of a highly sensitive search strategy recommended for identifying randomized trials. No limits were used in any search. Additionally, professional journals check details and proceedings of meetings were screened, and nationally recognized experts in the field of pediatric pain management were asked for further sources of data.
Selection criteria: Randomized, controlled trials comparing the use of a continuous infusion of epidural local anesthetics plus intravenous opioids vs. intravenous opioids only for postoperative pain management in adolescent scoliosis repair were eligible for inclusion in the meta-analysis. All studies had to include at least the primary outcome of interest, postoperative pain scores.
Data collection and analysis: After the development of a data collection and extraction form, two independent reviewers extracted all. No data conflicts were encountered.