Leuprolide acetate treatment of mice bear ing U118 MG tumors triggered as much as 90% growth inhibition in contrast with placebo for 144 days. U87 MG tumors had been development inhibited by leuprolide acetate by as much as 68% for somewhere around 60 days. To our know-how, there are actually no published reviews that leuprolide acetate has development inhibitory effects in GBM. Clinical trials are planned to determine the efficacy of leuprolide acetate in extending disorder zero cost survival just after tumor resection in GBM patients. ET eleven. CPT 11, A Potential NEW CHEMOTHERAPY DRUG FOR ATYPICAL OR MALIGNANT MENINGIOMA Vinay Gupta,1 Yuzhuang S. Su,two Christian G. Samuelson,three Florence M. Hofman,1,2 Axel H. Sch?nthal,4 and Thomas C. Chen1,two,five,six, one Departments of Pathology, 2Neurosurgery, 4Molecular Microbiology and Immunology, 5K. Norris Jr.
Detailed Cancer Center, University of Southern California, selleck Los Angeles, CA, USA, and 3Temple University School of Medication, Temple University, Philadelphia, PA, USA While DMXAAA most meningiomas are treated surgically, atypical or malig nant meningiomas and surgically inaccessible meningiomas may possibly not be eliminated wholly and have a tendency to recur usually. We established the effects with the topoisomerase I inhibitor, CPT 11, on primary meningioma cultures and also a malignant meningioma cell line in vitro and in vivo. The results of CPT eleven on cellular proliferation in principal meningioma cultures along with the IOMM Lee malignant meningioma cell line have been measured by an MTT assay and flow cytometry examination. Apoptosis immediately after drug therapy was evaluated by TUNEL as well as the DNA laddering assay. The results of CPT 11 in vivo inside a meningioma model have been deter mined having a subcutaneous murine tumor model implementing the IOMM Lee cell line.
CPT 11 induced a dose dependent anti proliferative

effect, with sub sequent apoptosis in key meningioma cultures plus the IOMM Lee human malignant meningioma cell line. In our animal model, CPT eleven remedy led to a statistically significant decrease in tumor development. An HPLC examination demonstrated conversion of CPT 11 to the active metabolite SN 38 in tumor specimens. Treatment method was accompa nied by a decrease in Bcl 2 and survivin levels and an increase in apoptotic cell death. CPT 11 inhibited meningioma growth both in vitro and in vivo. CPT 11 was much more effective against the malignant meningioma cell line than against principal meningioma cultures. Therefore, this drug may perhaps have an important therapeutic role in the treatment of atypical or malignant meningiomas and should be evaluated further for this purpose. ET 12. CHEMOTHERAPY FOR MENINGIOMAS WITH GLEEVEC BOTH IN VIVO AND IN VITRO Vinay Gupta,1 Yuzhuang S. Su,2 Christian G. Samuelson,three Florence M. Hofman,one,2 Axel H. Sch?nthal,4 and Thomas C.