Tplain was positioned in the top #selleck inhibitor randurls[1|1|,|CHEM1|]# left quadrant. Figure 3 PCA plot showing the clustering of the samples. The figure shows a PCA plot based on taxonomic (phylum level) and metabolic (SEED subsystems, level I) parameters combined. The geochemical

[25] parameters were overlain using the envfit function of the vegan library in R. The first principal components accounted for 95 % of the variation in the dataset, while the second principal component accounted for 3 %. All metagenome data were given as percent of total reads. The geochemical parameters were normalized by dividing with the standard deviation and subtracting the smallest number from all numbers in each row. Plot A: the metagenomic parameters are represented by red arrows. Labels are shown for parameters with Euclidian distance over 0.1 from origin. The geochemical parameters are represented by blue arrows. Only the most significant geochemical parameters are shown (p-value < 0.1). Plot B: is an excerpt of plot A, magnifying the central region of the plot. Labels for all metagenomic parameters with Euclidian distance over 0.02 are included. The first principal component (PC1) accounted for 95% of the variance in the dataset. Along the PC1 axis Tpm2 was the Troll sample most similar to the Oslofjord samples, while Tplain and Tpm1-2 were positioned furthest away. Tpm3 and Tpm1-1 were placed at an intermediate position. The

abundance of Proteobacteria was the most important parameter for the positioning of sites along PC1. Proteobacteria, as well as Thaumarchaeota, Planctomycetes Bromosporine solubility dmso and Actinobacteria had high negative scores along this axis. The analysis thereby indicated relatively high abundances of these taxa at the sites placed on the left side of the plot, especially Tpm1-2 and Tplain (Figure 3, Additional file 5: Table S3). Firmicutes, Euryarchaeota, Chloroflexi and Viruses all had high positive scores along PC1 indicating that the samples placed in the right section of the PCA plot (OF1, OF2 and Tpm2) had relatively high abundances of these taxa compared to the other sites. Although

Tpm2 grouped with the Oslofjord Rucaparib in vitro samples along PC1, it was separated from the Oslofjord samples by PC2. While Chloroflexi, Euryarchaeota, Thaumarchaeota and Firmicutes had high negative scores along PC2, Bacteroidetes, Actinobacteria and Planctomycetes had the highest positive scores along this axis and can therefore be considered as important parameters for the placement of the Oslofjord samples and Tplain in the top half of the plot. Concerning the carbon sources, the geochemical parameters supported a positive correlation between hydrocarbons (< n-C32) and the Troll samples, while concentrations of bicarbonate and TOC were positively correlated with the Oslofjord samples (Figure 3, Additional file 4: Table S2 and Additional file 6: Figure S3).