Fig. S6 Dengue serotype 2 complete E gene analysis. The phylogeny was inferred by Neighbor-joining method. The optimal tree is shown. Strains obtained during the study are marked in bold. Fig. S7 Dengue serotype 3 complete E gene analysis. The phylogeny was inferred by Neighbor-joining method. The optimal tree is shown. Strains obtained during the study are marked in bold. Fig. S8 Dengue serotype 4 complete E gene analysis. A: Neighbor-joining method. The optimal tree (sum of branch length = 0.61297211) is shown. B: Maximum-likelihood method. The tree with the highest log (−8058.5260) is shown. 116 nucleotide sequences were

included RO4929097 in the analysis. “
“Background. The etiological spectrum of cerebro-meningeal infections (CMI) in travelers has never been specifically analyzed. Objectives. To assess the etiologies of CMI in hospitalized travelers and to propose a diagnostic approach to travel-related CMI. Methods. During an 8-year period, we retrospectively collected data on all travelers hospitalized in our department for a CMI occurring during travel or in the month after their return. Results. Fifty-six patients (35 men and 21 women; mean age 29 years (16–83); 44.6% tourists, 26.8% military, 16% immigrants, 12.5% expatriates) CYC202 ic50 were included. The main destinations were Africa (57.2%), Europe (19.5%), and Asia (12.5%). The median duration of travel was

24 days (5–550). Symptoms occurred during travel in 20 patients (11 of which required a medical evacuation). In the remaining 36 patients, the median duration between return and clinical onset was 10 days. The median time from clinical onset to hospitalization was 4 days (0.5–96). Twenty-four patients presented with a meningeal syndrome and 20 others

with encephalitic features. The remaining 12 patients had an incomplete clinical presentation (headaches or fever). The etiology was confirmed in 42 cases (75%) of which tropical diseases (n = 14) were less common than cosmopolitan ones (n = 28). Sub-Saharan Plasmodium falciparum malaria (n = 12) was the leading tropical infection, whereas viral infections (enterovirus, herpesviridae, HIV) were the main cosmopolitan etiologies. Only four bacterial infections Sinomenine were reported (Neisseria meningitidis, Mycoplasma pneumoniae, Brucella melitensis, Salmonella typhi). Sixteen patients were admitted to intensive care for a median time of 9.5 days (1–63). The average duration of hospitalization was 14 days (3–63). One death by herpes simplex virus 1 encephalitis was recorded. Four patients (7%) had neurological sequelae. Conclusions. Among the diversified etiological spectrum of CMI, cosmopolitan infections are widely predominant, particularly viral infections, followed by tropical causes, of which malaria is the leading disease in returnees from endemic areas. The diagnostic approach should be driven by history and physical examination.