Thus, we manually analysed the differentially displayed genes involved in these processes. We have identified two probe sets Mtr. 30770. 1. S1 at Mtr. 10439. 1. S1 at that are homologues to Arabidopsis ETHYLENE INSENSITIVE3. These two probe sets were down regulated 2. 6 fold and cisplatin dna 1. 8 fold respectively, In contrast, a response regulator was up regulated the embryogenic 2HA cultures. This was con firmed by real time RT PCR. Comparison of gene expression between the embryogenic cultures and seed development To identify common genes expressed between embryo genic Inhibitors,Modulators,Libraries cultures and developing seeds, we have compared our data to that from the Medicago Expression Atlas. We have chosen developing seeds at 10 days after pollination since this is the earliest time point available for seed devel opment in the Atlas and contrasted it to leaf.

Inhibitors,Modulators,Libraries A total of 12,954 probe sets showed differentially display between the developing seed at ten days after pollination and leaf samples. Over 6,800 probe sets were up regulated in the developing seeds at least two fold, of which 14 were also up regulated Inhibitors,Modulators,Libraries in the embryogenic cultures when compared to non embryogenic cultures. Over 6,000 probe sets were down regulated in the developing seeds at least two fold, of which 6 were also down reg ulated in the embryogenic cultures when compared to non embryogenic cultures. these include transcription factor EIL1, a H transporting ATPase Mtr. 5635. 1. S1 at Snakin like cysteine rich protein, a patatin like phospholipase, a thaumatin like protein and a hypothetical protein.

In brief, we have identified a small number probe sets that were either up or down regulated in both the embryogenic cultures and the developing Inhibitors,Modulators,Libraries seeds. These include transcription factors such as response regulator MtRR1 and EIN3, nodulins and unknown pro teins. Further investigation of these pro teins Inhibitors,Modulators,Libraries will shed light on the similarities between somatic and zygotic embryogenesis. Comparison between the array and proteomics We also compared our array data with the proteome data obtained for the explant leaf cultures of 2HA and Jemalong. 16 protein spots were reportedly identified as differentially displayed proteins between the explant leaf cultures of 2HA and Jemalong after 2, 5 and 8 weeks of culture. Although all of the corresponding genes were present on the array, none of them showed differential display when used 2 fold cut off and student t test.

Thus, we were not able to find any correla tion between transcriptomics and proteomics of the explant leaf cultures of 2HA and Jemalong. This probably due to the fact that only a very limited number of selleck differ entially displayed proteins were identified by proteomics, most of which showed differential display only at the later stages of culture but not at the early stage at which this microarray analy sis was focused on.