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“Study of interaction between drugs and metals is an active research area in bioinorganic chemistry. Interactions between drug and metal may inadvertently reduce or increase the drug effect.1 Amlodipine besylate is a widely used anti-hypertensive drug. It selectively inhibits calcium influx across cell membranes in cardiac and vascular smooth muscle. It is a peripheral arteriolar vasodilator; selleck screening library thus it reduces

after load.2 It is useful for the treatment of angina, essential hypertension, congestive heart failure and Reynaud’s disease.3 Calcium is the 5th most abundant element in the body and the major fraction is in the bony structure. Calcium plays important physiological roles in the maintenance of the functional integrity of the nervous, muscular and skeletal systems, cell membrane

and capillary permeability. Protein binding generally refers to the binding of a drug to plasma proteins. The amount of drug bound to protein determines how effective the drug is in MK 1775 the body. Serum albumin, the most abundant protein in the blood, plays a very important role in the binding phenomenon and serves as a depot and transport protein for numerous endogenous compounds.4 Among the plasma protein, albumin is mostly bounds to ligands or drug. Since number of protein binding sites is limited, competition will exist between two drugs and the drug with higher affinity will displace the other, causing increased free drug concentration, which leads to higher toxicity or short duration of action of the related drug.5 The ability of one drug to inhibit the other is a function of their relative concentration, binding affinities and specifically of binding.6

BSA and HSA have structural similarity.7 In this study BSA is in lieu of HAS, because of low cost and easy availability. This study was aimed to evaluate interaction of Amlodipine besylate with (Ca2+) present in multi-vitamins and foods as well as influence of (Ca2+) on protein binding of drug. Amlodipine besylate were given by Square Pharmaceuticals Ltd., Bangladesh, Bovine serum albumin (Fatty acid free, fraction V, 96–98%, Sigma) and Dialysis Membrane (Medicell, England) and Calcium chloride and all other reagents were purchased from Merck, India. Tryptophan synthase Following methods were used for the commencement of the experiment: Initial detection of complexation of Amlodipine besylate and Ca2+ had done from the nature of spectra of pure drug as well as their 1:1 mixtures in buffer solutions of pH 1.2, 2.2, 6.4, 7.4 at a fixed concentration (0.1 × 10−4) M were compared with those of each interacting species.8 The various concentrations of the samples were kept at very dilute levels in each case and recorded between 300 and 400 nm using a UV–VIS automatic recording instrument with a constant temperature cell compartment and automatic recording unit. This study was done by method of Vogel.