Both Polymyxa graminis and Polymyxa betae were identified This i

Both Polymyxa graminis and Polymyxa betae were identified. This is the first report of infection of Arabidopsis by Polymyxa spp. and shows the possibility check details of using this system for studies of infection biology and host–parasite interactions. Polymyxa spp. are a group of obligate root-infecting organisms belonging to the plasmodiophorid group that are important plant–virus vectors (Kanyuka et al., 2003). Polymyxa graminis transmits viruses such as soil-borne cereal mosaic virus (SBCMV), soil-borne wheat mosaic virus and wheat spindle streak mosaic virus to cereals. Polymyxa

betae transmits beet necrotic yellow vein virus, the cause of rhizomania, to sugar beet. Polymyxa graminis has a wide host range including wheat, barley, rye, rice, sorghum, groundnut and various grasses, whereas P. betae is mostly restricted to beet and other plants in the family Chenopodiaceae. A number of subgroups (ribotypes) of Polymyxa spp. have been identified according to rDNA sequence data (Ward et al., 1994, 2005; Ward & Adams, 1998; Legrève et al., 2002). Some of the P. graminis ribotypes appear to differ in host range and temperature requirements, leading to the suggestion that they should be classified as formae speciales (Legrève et al., 1998, 2002). Two groups of P. graminis isolates are found in temperate regions: ribotype I (f. sp. temperata) and ribotype II (f. sp. tepida). All selleck chemicals llc internal transcribed

spacer (ITS) rDNA sequences Carbachol for P. betae reported to date fall

into two types that differ by only one base pair (Ward & Adams, 1998; Legrève et al., 2002). Because of their obligate nature and relatively long life cycle, Polymyxa spp. have been difficult to study. The development of a model system for studying Polymyxa–plant interactions would be extremely useful. Arabidopsis thaliana is an invaluable model system for several reasons: (1) short generation time, (2) the ability to grow large numbers in a relatively small space, (3) its ability to self-fertilize, (4) the large number of progeny that can be produced from a single plant, (5) its small haploid genome containing a relatively small number of repetitive genetic elements, (6) the availability of a fully sequenced genome, (7) the availability of mutagenized lines, (8) ease of transformation and (9) the large number of ecotypes exhibiting natural variation available (Meyerowitz, 1989). These features are in contrast to many crop species such as cereals, where genetic resources are less well advanced. Arabidopsis has already been used very successfully to study the interactions of another plasmodiophorid: Plasmodiophora brassicae (Koch et al., 1991). The ability to separate host sequences from those of Plasmodiophora by bioinformatics analysis has simplified the interpretation of data, for example from suppressive subtractive hybridization experiments to study gene structure and expression (Bulman et al., 2006, 2007).

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