Patients with proteinuria (urine dipstick ≥2+), impaired creatinine clearance (<30 ml/min), or abnormal serum calcium levels (>2.75 or <2.08 mmol/l) at screening were not eligible for study participation. Patients with an ongoing infection (including dental infection) or planned oral surgery within 3 months of randomization were also excluded. Study medications All patients received an infusion of ZOL 5 mg over at

least 15 min on Day 1. Patients were randomized to one of three treatment groups. All Selleck Capmatinib oral study drugs were double-blinded with matching placebo capsules. In Group 1, 45 min prior to ZOL infusion, two capsules of acetaminophen 325 mg were administered orally. Subjects in this group continued to take two capsules of acetaminophen four times daily for the

next 3 days at home. In Group 2, 45 min prior to ZOL infusion, two capsules of immediate-release fluvastatin 40 mg were administered orally. Fluvastatin was administered only once, on Day 1, prior to ZOL infusion. Subjects in the fluvastatin group were provided with placebo capsules (matching acetaminophen) and took two capsules four times daily for the next 3 days at home. In Group 3, subjects received placebo 45 min prior to ZOL infusion and continued to take two capsules of placebo (matching acetaminophen) four times daily for the next 3 days at home. Patients in this study were also provided with calcium 600 mg plus vitamin D3 400 IU (one tablet twice daily). Open-label rescue Geneticin medication (ibuprofen 200 mg Baf-A1 cost tablets Tideglusib chemical structure every 4 to 6 h, not to exceed eight tablets in a 24-h period) could be taken if the patient had an oral body temperature ≥38.9°C (102°F) and/or severe symptoms of fever, headache, myalgia, or arthralgia. Study personnel observed each patient ingest the first dose of study medication and receive the ZOL

infusion. Patient diaries and unused medication were used to assess compliance during the remainder of the study. Patients recorded the dose, date, and time of study and rescue medication use in diaries and returned used bottles and unused study and rescue medication at the final visit. Efficacy and safety variables The primary efficacy variable in this study was the proportion of patients who had a clinically significant increase in oral body temperature (≥1°C from baseline and ≥38.5°C overall) or used rescue medication at least once during the 3-day period following ZOL infusion. Oral body temperature was measured at baseline prior to ZOL infusion using a digital thermometer (Welch Allyn Sure Temp), which was provided to each patient for the duration of the study. Patients were trained to take two temperature assessments within 10 min of each other four times per day for 3 days. Temperature assessments were conducted after completing VAS and symptom questionnaires and prior to taking any oral study medication.