7A, upper panel) The relative numbers of Ki67-positive cells (in

7A, upper panel). The relative numbers of Ki67-positive cells (in uninfected cells and HCV1a-infected cells with and without buy Atezolizumab DLC-1 cDNA transfection) is shown in Fig. 7B. In addition, we quantified the enhanced proliferative capacity of HCV1a-infected primary hepatocytes by way of fluorescence-activated cell sorting analysis of Ki67-labeled cells (Fig. 7C). The results showed an approximately 40% increase in cell proliferation of HCV1a-infected

hepatocytes (3 days posttransfection). The increased cell proliferation is neutralized when the DLC-1 level was artificially increased through transfection with a DLC-1 expression vector. Recent studies support the oncogenic role of miRNAs in different human neoplasms including HCC,30 glioblastoma,31 urinary bladder cancer,32 papillary learn more tumors of the thyroid,33 and pancreatic cancer.34 However,

the role of miRNA-mediated oncogenesis remains unclear for HCV infection. We present several lines of evidence that HCV infection results in the induction of miR-141, which silences DLC-1 expression, a tumor suppressor gene that is frequently deleted in HCC and other solid human tumors. The results presented in this study support a link between HCV replication and altered expression of miR-141 that target a tumor suppressor gene frequently deleted in HCC. Tumor suppressor genes can influence oncogenic virus replication by negatively regulating pro-oncogenic signaling proteins.6 NF1 inhibits the Ras signaling pathway, which is deregulated in many cancers and can be a potential therapeutic target. Phosphatase and tensin homologue inhibits the phosphoinositide 3-kinase (PI3K) pathway, and inhibitors of PI3K components such as PI3K, AKT, and mTORs have been similarly pursued for cancer therapy.11 The results presented here support a model of HCV-associated hepatocarcinogenesis, based on miRNA-mediated 上海皓元医药股份有限公司 silencing of tumor suppressor DLC-1. The intracellular induction of miR-141 by HCV

appears to translationally inhibit the tumor suppressor DLC-1, whose depletion promotes cell proliferation. Although our findings support the role of DLC-1 in HCV-associated hepatocarcinogenesis, they do not by themselves support a direct role of DLC-1 in regulating HCV replication, nor do they rule out possible contribution of other tumor suppressor genes. Dependence of HCV replication on miRNAs has been debated in earlier studies.18, 19, 30 In recent studies, Fornari et al.4 have presented evidence that miRNA-221 induced in HCC tissues promotes tumorigenesis by targeting the CDK inhibitors CDKN1C/p57 and CDKN1B/p27. Our findings support the argument that miR-141–targeted suppression of tumor suppressor DLC-1 may promote the initial stages of HCV-associated hepatocarcinogenesis and cell proliferation.

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